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pH 依赖性荧光 Keima 对流式细胞术 Mitophagy 的敏感性测定
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Biologia
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JoVE Journal Biologia
Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima
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pH 依赖性荧光 Keima 对流式细胞术 Mitophagy 的敏感性测定

DOI:
10.3791/58099-v

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09:13 min

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August 12, 2018

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, , ,
1Peripheral Neuropathy Research Center, College of Medicine,Dong-A University, 2Department of Biochemistry, College of Medicine,Dong-A University, 3Aging Institute, Department of Medicine,University of Pittsburgh School of Medicine

Capítulos

  • 00:00Título
  • 01:02Generation of Cells Expressing mt-Keima
  • 03:25Inducing Mitophagy using CCCP Treatment and Preparing FACS Samples
  • 04:29FACS Analysis of Mitophagy
  • 07:16Results: Analysis of CCCP-induced Mitophagy in HeLa-mt-Keima Cells by Flow Cytometry
  • 07:55Conclusion

Summary

Tadução automática

Tadução automática

Mitophagy, 线粒体的选择性降解, 与线粒体稳态有关, 在各种人类疾病中被解除管制。然而, 方便的实验方法测量 mitophagy 活动是非常有限的。在这里, 我们提供了一个敏感的检测 mitophagy 活动使用流式细胞仪。

Tags

Keywords: MitophagyFlow CytometryMt-KeimaCCCPHeLa CellsMitochondriaLentivirusPuromycin

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