Journal
/
Bioquímica
/
Carboxylesterases 在抗杀虫剂家蝇、家蝇中的功能表征
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica
Please note that all translations are automatically generated. Click here for the English version.

Carboxylesterases 在抗杀虫剂家蝇、家蝇中的功能表征

DOI:
10.3791/58106-v

•

08:17 min

•

August 23, 2018

•

,
1Department of Entomology and Plant Pathology,Auburn University

Capítulos

  • 00:04Título
  • 00:53Expression and Isolation of Target Proteins with a Baculovirus-mediated Insect Cell Expression System
  • 04:18A Cell-based MTT Cytotoxicity Assay
  • 06:30Results: MdαE7 Expressing Cells Show Higher Viability when Exposed to Permethrin
  • 07:12Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出了一个协议, 生产的房子飞羧酸蛋白的体外与杆状病毒介导的昆虫细胞表达系统, 后来功能表征其在代谢氯菊酯的作用, 从而, 赋予以传导细胞为基础的 mtt 法和体外代谢研究的抗性。

Tags

Keywords: CarboxylesterasesInsecticide ResistanceHouse FliesMusca DomesticaFunctional CharacterizationInsect ToxicologyProtein ExpressionIn Vitro AssayBaculovirus Expression SystemSF9 CellsGFPGanciclovir

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58106?article_type=v

植物甘露聚糖细胞壁多糖的结构表征
check_url/pt/58106?article_type=v

改进的小鼠骨骼肌染色质沉淀的协议
check_url/pt/58106?article_type=v

用 X 射线晶体学和生物物理技术表征免疫球蛋白折叠的糖蛋白
check_url/pt/58106?article_type=v

聚丙烯酰胺凝胶中蛋白质的荧光银染色
check_url/pt/58106?article_type=v

用奇米蛋白构建功能蛋白区的鉴定
check_url/pt/58106?article_type=v

大肠杆菌中异构表达对膜迁移者的特性与膜囊泡的产生
check_url/pt/58106?article_type=v

SUMO 结合实体 （SUBEs） 作为肝癌中 SUMO 蛋白酶的浓缩、分离、识别和表征的工具
check_url/pt/58106?article_type=v

RING-E3型无二体气体在Vitro和Planta中的功能特性
check_url/pt/58106?article_type=v

使用荧光寿命成像在老化C.Elegans中淀粉样蛋白结构的表征
check_url/pt/58106?article_type=v

实时测量核苷酸与完整功能性膜蛋白的结合

Read Article