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衣藻中心粒单粒子重建的分离与荧光成像
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
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衣藻中心粒单粒子重建的分离与荧光成像

DOI:
10.3791/58109-v

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10:38 min

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September 21, 2018

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, , , , ,
1Department of Cell Biology, Sciences III,University of Geneva

Capítulos

  • 00:04Título
  • 00:46Purification of C. reinhardtii Centrioles
  • 05:15Concentration of Centrioles onto the Center of the Coverslips
  • 07:06Quantification of Isolated Centrioles on Coverslips: Centrifugation and Immunofluorescenc
  • 08:47Results: Isolation and Fluorescence Imaging of Chlamydomonas Centrioles
  • 10:03Conclusion

Summary

Tadução automática

Tadução automática

我们已经制定了一个战略, 以净化和图像大量的中心粒在不同的方向, 适合超分辨率显微镜和单粒子平均。

Tags

Keywords: CentrioleChlamydomonasSingle-particle ReconstructionFluorescence ImagingIsolationProtein LocalizationDeflagellationSucrose CushionCentrifugationPBSLysis Buffer

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