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En proksimal kultur metode til at studere Paracrine signaler mellem celler
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Biologia
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JoVE Journal Biologia
A Proximal Culture Method to Study Paracrine Signaling Between Cells

En proksimal kultur metode til at studere Paracrine signaler mellem celler

DOI:
10.3791/58144-v

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08:17 min

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August 28, 2018

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, , , ,
1Indiana University School of Medicine, 2Urology,Indiana University Health Southern Indiana Physicians, 3Indiana University Melvin and Bren Simon Cancer Center, 4Department of Medical and Molecular Genetics,Indiana University School of Medicine

Capítulos

  • 00:04Título
  • 01:01Proximal Culture Setup
  • 03:46RNA Extraction
  • 05:37Results: Representative Proximal Culture Effects on mRNA Expression
  • 07:31Conclusion

Summary

Tadução automática

Tadução automática

Paracrine og juxtacrine cellulær interaktioner spiller en vigtig rolle i mange biologiske processer, herunder tumor progression, immunrespons, angiogenese og udvikling. Her, er en proksimal kultur metode brugt til at studere paracrine signalering hvor de lokaliserede koncentrationer af de faktorer, der udskilles vedligeholdes samtidig forhindre direkte cellulære kontakt.

Tags

Paracrine SignalingIntercellular CommunicationProximal Culture MethodCancer CellsTumor MicroenvironmentOvarian Cancer CellsHPMCJuxtacrine SignalingCell CultureCell Interactions

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