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Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
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Capítulos
- 00:04Título
- 00:51Patterned Sapphire Disc Preparation, Cell Culture, and Imaging
- 03:16Cryo-immobilization and Freeze Substitution (FS)
- 05:24Resin Embedding and Patterned Sapphire Disc Removal from the Polymerized Resin Block
- 06:43Ultrathin Section Preparation and Imaging via Transmission Electron Microscopy (TEM)
- 08:06Results: Tracking and Imaging of Viral Protein-associated Structures via Correlative Light Electron Microscopy (CLEM)
- 08:47Conclusion
A correlative light electron microscopy (CLEM) method is applied to image virus-induced intracellular structures via electron microscopy (EM) in cells that are previously selected by light microscopy (LM). LM and EM are combined as a hybrid imaging approach to achieve an integrated view of virus-host interactions.
