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低温固定化细胞跟踪和成像病毒蛋白相关结构的相关光电子显微镜 (克莱)

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

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低温固定化细胞跟踪和成像病毒蛋白相关结构的相关光电子显微镜 (克莱)

DOI:

10.3791/58154-v

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09:18 min

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September 07, 2018

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Rachel Santarella-Mellwig, Uta Haselmann, Nicole L. Schieber, Paul Walther, Yannick Schwab, Claude Antony, Ralf Bartenschlager⁴, Inés Romero-Brey

¹European Molecular Biology Laboratory, ²Department of Infectious Diseases, Molecular Virology,Heidelberg University, ³Central Facility for Electron Microscopy,Ulm University, ⁴Heidelberg Partner Site,German Center for Infection Research

Capítulos

00:04Título
00:51Patterned Sapphire Disc Preparation, Cell Culture, and Imaging
03:16Cryo-immobilization and Freeze Substitution (FS)
05:24Resin Embedding and Patterned Sapphire Disc Removal from the Polymerized Resin Block
06:43Ultrathin Section Preparation and Imaging via Transmission Electron Microscopy (TEM)
08:06Results: Tracking and Imaging of Viral Protein-associated Structures via Correlative Light Electron Microscopy (CLEM)
08:47Conclusion

Summary

Tadução automática

采用相关光电子显微镜 (克莱) 方法, 通过光学显微术 (LM) 在细胞内的电子显微术 (EM) 对病毒诱导的胞内结构进行图像分析。LM 和 EM 作为一种混合成像方法, 实现了病毒宿主交互的集成视图。

Tags

Correlative Light Electron Microscopy (CLEM)Viral Protein Cellular Membranes Bio-replication High Pressure Freezing Cryo-immobilization Electron Microscopy Light Microscopy Fluorescent Proteins Virus-infected Cells Transfected Cells Ultrastructure Analysis

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