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Hyaluronsyre basert Hydrogels for 3-dimensjonale kultur pasient-avledede glioblastom celler
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JoVE Journal Bioengenharia
Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

Hyaluronsyre basert Hydrogels for 3-dimensjonale kultur pasient-avledede glioblastom celler

DOI:
10.3791/58176-v

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08:35 min

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August 24, 2018

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, , ,
1Department of Bioengineering,University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute,University of California, Los Angeles

Capítulos

  • 00:04Título
  • 00:31Hydrogel Crosslinking and Cell Encapsulation
  • 03:11Hydrogel Culture Single Cell Extraction for Flow Cytometry and Cryopreservation for Sectioning
  • 06:10Results: Hyaluronic Acid-Based 3D Patient-Derived Glioblastoma Cell Hydrogel Cultures
  • 07:55Conclusion

Summary

Tadução automática

Tadução automática

Her presenterer vi en protokoll for tredimensjonale kultur av pasient-avledede glioblastom celler i ortogonalt tunable biologisk materiale designet for å etterligne brain matrix. Denne tilnærmingen gir en i vitro, eksperimentelle plattform som opprettholder mange egenskaper i vivo glioblastom celler vanligvis mistet i kultur.

Tags

Hyaluronic AcidHydrogels3D Cell CultureGlioblastomaExtracellular MatrixPatient-derived CellsCell EncapsulationPEG-MalSilicone Rubber MoldsCell DissociationCell SuspensionGelation

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