Journal
/
Pesquisa do Câncer
/
Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence
JoVE Journal
Pesquisa do Câncer
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Pesquisa do Câncer
Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

Methods for Evaluating the Role of c-Fos and Dusp1 in Oncogene Dependence

DOI:
10.3791/58194-v

•

10:09 min

•

January 07, 2019

•

, ,
1Cancer Blood Disease Institute, Divisions of Experimental Hematology and Cancer Pathology,Cincinnati Children’s Hospital Medical Center

Capítulos

  • 00:04Título
  • 00:48Isolation of c-Kit+ Cells from Bone Marrow
  • 03:52Transduction
  • 06:43Colony-forming Unit Assays
  • 08:25Results: c-Fos and Dusp1 Therapeutic Target in Leukemia
  • 09:36Conclusion

Summary

Tadução automática

Tadução automática

Here, we describe protocols for the genetic and chemical validation of c-Fos and Dusp1 as a drug target in leukemia using in vitro and in vivo genetic and humanized mouse models. This method can be applied to any target for genetic validation and therapeutic development.

Tags

Keywords: C-FosDusp1Oncogene DependenceCancer TherapeuticsLeukemiaSolid TumorsLung CancerBrain TumorsPancreatic TumorsBone MarrowMouseCell IsolationCD117MACSCell SeparationCell Culture

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58194?article_type=v

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
check_url/pt/58194?article_type=v

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
check_url/pt/58194?article_type=v

Evaluating In Vitro DNA Damage Using Comet Assay
check_url/pt/58194?article_type=v

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
check_url/pt/58194?article_type=v

Evaluating the Role of Mitochondrial Function in Cancer-related Fatigue
check_url/pt/58194?article_type=v

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
check_url/pt/58194?article_type=v

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
check_url/pt/58194?article_type=v

Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
check_url/pt/58194?article_type=v

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
check_url/pt/58194?article_type=v

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

Read Article