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A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
 

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

Article DOI: 10.3791/58208-v • 05:36 min • September 6th, 2018
September 6th, 2018 •
1, 1
1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

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A long-term culture model of bovine granulosa cells under serum-free conditions is described. This model allows researchers to study the effects of diverse factors and conditions as different plating densities on the characteristics of estrogen-producing bovine granulosa cells.

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Keywords: Tissue Culture Estrogen-producing Primary Bovine Granulosa Cells Cryopreservation Cell Isolation Follicular Fluid Granulosa Cells Cell Culture RNA DNA Protein Analysis Cell Freezing Cell Thawing Alpha MEM
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