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Imunologia e Infecção

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动态粘附法在炎症性肠病抗粘连治疗功能分析中的作用

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

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动态粘附法在炎症性肠病抗粘连治疗功能分析中的作用

DOI:

10.3791/58210-v

•

08:27 min

•

September 20, 2018

•

Emily Becker, Sebastian Schramm, Marie-Theres Binder, Clarissa Allner, Maximilian Wiendl, Clemens Neufert, Imke Atreya, Markus Neurath, Sebastian Zundler

¹Department of Medicine 1,University of Erlangen-Nuremberg

Capítulos

00:04Título
00:45Capillary and Cell Preparation
03:54Live Cell Imaging of Dynamic Adhesion and Evaluation
06:52Results: Representative Human PBMC Adherence Assessment
07:33Conclusion

Summary

Tadução automática

免疫细胞对血管壁的动态黏附是肠道归巢的前提。在这里, 我们提出了一个功能性的体外检测的协议, 以影响分析抗整合素抗体, 趋化因子或其他因素的细胞动态细胞黏附的人体细胞使用 addressin 涂层毛细血管。

Tags

Dynamic Adhesion Assay Anti-adhesion Therapies Inflammatory Bowel Disease Cell Adhesion Flow Conditions Addressin Peripheral Blood Mononuclear Cells Cell Tracking Dye

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