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Neurogenesis Using P19 Embryonal Carcinoma Cells
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Neurogenesis Using P19 Embryonal Carcinoma Cells

Neurogenesis Using P19 Embryonal Carcinoma Cells

DOI:
10.3791/58225-v

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07:46 min

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April 27, 2019

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, , , , , , ,
1Department of Experimental Embryology, Institute of Genetics and Animal Breeding,Polish Academy of Sciences, 2Department of Regenerative Medicine,Maria Skłodowska-Curie Institute – Oncology Center, 3Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding,Polish Academy of Sciences

Capítulos

  • 00:04Título
  • 00:27Culture Maintenance and Sub-culturing Cells
  • 02:13Trypsin Digestion
  • 03:29Aggregate Generation
  • 04:34Aggregate Dissociation
  • 05:47Plating Cells
  • 06:31Results: Neuronal Differentiation of P19 Cell Line
  • 07:23Conclusion

Summary

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The P19 mouse embryonic carcinoma cell line (P19 cell line) is widely used for studying the molecular mechanism of neurogenesis with great simplification compared to in vivo analysis. Here, we present a protocol for retinoic acid-induced neurogenesis in the P19 cell line.

Tags

Keywords: P19 Embryonal Carcinoma CellsNeurogenesisNeuron DifferentiationCell CultureTrypsin DigestionMaintenance MediumDifferentiation Medium

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