/
/
Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Título
- 00:44Human Primary and Expanded Natural Killer (NK) Cell Transduction
- 04:48Results: Representative Electroporation Efficiency
- 06:13Conclusion
Here, we present a protocol to genetically modify primary or expanded human natural killer (NK) cells using Cas9 Ribonucleoproteins (Cas9/RNPs). By using this protocol, we generated human NK cells deficient for transforming growth factor–b receptor 2 (TGFBR2) and hypoxanthine phosphoribosyltransferase 1 (HPRT1).
