Journal
/
Imunologia e Infecção
/
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

DOI:
10.3791/58255-v

•

07:10 min

•

January 07, 2019

•

, , , ,
1Department of Molecular and Cellular Biology,Beckman Research Institute of City of Hope, 2Irell and Manela Graduate School of Biological Sciences,Beckman Research Institute of City of Hope

Capítulos

  • 00:04Título
  • 00:41Intrahepatic Hematopoietic Stem Cell (HSC) Injection
  • 01:59HSC Engraftment Validation
  • 03:48HIV Infection Analysis and Viral Suppression and Rebound Validation
  • 05:20Results: Engraftment Validation and HIV Infection and Suppression Analyses
  • 06:26Conclusion

Summary

Tadução automática

Tadução automática

This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.

Tags

Hu-NSG Mouse ModelHIV ReplicationHIV LatencyNon-invasiveHuman Stem CellsCD34+ HSCsNOD/SCID/IL2rγnull MiceCARTHIV InfectionEngraftment ValidationFlow Cytometry

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58255?article_type=v

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
check_url/pt/58255?article_type=v

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
check_url/pt/58255?article_type=v

Using the BLT Humanized Mouse as a Stem Cell based Gene Therapy Tumor Model
check_url/pt/58255?article_type=v

Humanized Mouse Model to Study Bacterial Infections Targeting the Microvasculature
check_url/pt/58255?article_type=v

Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
check_url/pt/58255?article_type=v

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
check_url/pt/58255?article_type=v

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
check_url/pt/58255?article_type=v

Influenza A Virus Studies in a Mouse Model of Infection
check_url/pt/58255?article_type=v

Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis
check_url/pt/58255?article_type=v

High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing

Read Article