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Journal / Developmental Biology / Immunohistochemical Detection of 5-Methylcytosine …
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
 

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Article DOI: 10.3791/58274-v • 07:50 min • August 29th, 2018
August 29th, 2018 •
1, 1, 1, 1
1BioTime, Inc

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The objective of this report is to describe the protocol for robust immunohistochemical detection of epigenetic markers, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in developing and postmitotic mouse retina.

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Keywords: Immunohistochemistry 5-methylcytosine 5-hydroxymethylcytosine Mouse Retina Retinal Development Chromatin Frozen Histological Preparation Cryosectioning Immunostaining DNA Methylation
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