/

Imunologia e Infecção

/

利用超滤离心技术分离小鼠支气管肺泡灌洗液中的细胞外囊

JoVE Journal
Imunologia e Infecção

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Imunologia e Infecção

Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique

Please note that all translations are automatically generated. Click here for the English version.

利用超滤离心技术分离小鼠支气管肺泡灌洗液中的细胞外囊

DOI:

10.3791/58310-v

•

09:18 min

•

November 09, 2018

•

Tanyalak Parimon, Norman E. Garrett III, Peter Chen², Travis J. Antes

¹Department of Medicine, Division of Pulmonary and Critical Care, Women’s Guild Lung Institute,Cedars-Sinai Medical Center, ²Department of Biomedical Sciences,Cedars-Sinai Medical Center, ³Department of Medicine, Smidt Heart Institute,Cedars-Sinai Medical Center

Capítulos

00:04Título
01:15Murine Bronchoalveolar Lavage Fluid (BALF) Collection and Preparation
02:21Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid
04:56Extracellular Vesicle Quantification
05:35Detection of BALF-derived Extracellular Vesicles
07:16Results: UFC Method Demonstrates Higher Total Particle Counts and Protein Recover
08:25Conclusion

Summary

Tadução automática

在这里, 我们描述了两个细胞外囊泡分离方案, 超滤离心和超离心与密度梯度离心, 从小鼠支气管肺泡灌洗液样品中分离细胞外囊泡。用两种方法对小鼠支气管肺泡灌洗液中的细胞外囊泡进行了定量和表征。

Tags

Extracellular Vesicles Bronchoalveolar Lavage Fluid Ultrafiltration Centrifugation Ultracentrifugation Density Gradient Purification Murine Trachea DPBS Supernatant Syringe Filter Centrifugal Filter Unit Retentate Aliquot

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

皮层小胶质细胞的分离与保存免疫表型和功能从新生儿鼠

口咽部气管内PAMP管理和支气管肺泡灌洗的利用率来评估小鼠宿主的免疫反应

鼠淋巴结基质细胞的分离

隔离和小鼠骨髓的静脉注射单核细胞衍生

从小鼠组织在母胎界面分离白细胞

分离及小鼠淋巴细胞的激活

流量Virometry来分析病毒颗粒的抗原谱和外囊泡

支气管肺泡灌洗小鼠肺分析炎症细胞浸润

成年日本血吸虫细胞外囊泡的分离与鉴定

用于阿尔韦拉尔巨噬细胞和CD4和T细胞免疫造血和HIV储液评估的支气管性洗浴液和匹配血液的处理

Read Article