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肾皮质细胞外基质衍生水凝胶的制备
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JoVE Journal Bioengenharia
Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
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肾皮质细胞外基质衍生水凝胶的制备

DOI:
10.3791/58314-v

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08:23 min

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October 13, 2018

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, , ,
1Department of Bioengineering,University of Washington, 2Department of Bioengineering, Center for Cardiovascular Biology, Institute for Stem Cell and Regenerative Medicine,University of Washington, 3Department of Medicine, Kidney Research Institute,University of Washington

Capítulos

  • 00:04Título
  • 00:25Preparation of Human Kidney Tissue
  • 01:39Isolation of Extracellular Matrix from Human Kidney
  • 03:16Fabrication of Hydrogel Stock Solution
  • 04:46Hydrogel Gelation
  • 06:15Results: Characteristics of Decellularized Tissue and Cell Growth in kECM
  • 07:44Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出一个协议, 制造一个肾脏皮层胞外基质衍生的水凝胶保留原生肾脏细胞外基质 (ECM) 结构和生化成分。介绍了制造工艺及其应用。最后, 讨论了利用该水凝胶支持肾脏特异细胞和组织再生和生物工程的观点。

Tags

Kidney CortexExtracellular MatrixHydrogelBiomaterialsCell Matrix InteractionsSulfateSDS SolutionRenal CapsuleMedullary RegionDecellularizationCell StrainerBleachHomogenization

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