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High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells

DOI:
10.3791/58351-v

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10:07 min

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January 07, 2019

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, ,
1Department of Microbial Infection and Immunity,The Ohio State University, 2Department of Microbiology,The Ohio State University, 3Infectious Diseases Institute,The Ohio State University, 4Division of Biostatistics, College of Public Health,The Ohio State University

Capítulos

  • 00:04Título
  • 00:31Cell Plating and Multi-plate Reader Imaging Cytometer Setup
  • 03:41High-throughput Fluorescence-based Assay and Cell Enumeration
  • 07:29Results: Representative Plasma Membrane Efficiency Resealing in Mammalian Cells
  • 09:15Conclusion

Summary

Tadução automática

Tadução automática

Here we describe a high-throughput fluorescence-based assay that measures the plasma membrane resealing efficiency through fluorometric and imaging analyses in living cells. This assay can be used for screening drugs or target genes that regulate plasma membrane resealing in mammalian cells.

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Keywords: Plasma Membrane ResealingHigh-throughputHeLa Cells96-well PlateFluorescencePropidium IodideKinetic AssayPlate ReaderOptical ConfigurationRead ModeRead Type

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