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Imunologia e Infecção

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双光子活体显微镜感病毒感染时气管黏膜的影像细胞相互作用

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

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双光子活体显微镜感病毒感染时气管黏膜的影像细胞相互作用

DOI:

10.3791/58355-v

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08:01 min

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August 17, 2018

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Miguel Palomino-Segura*^1,2, Tommaso Virgilio*^1,2, Diego Morone, Diego U. Pizzagalli³, Santiago F. Gonzalez

¹Faculty of Biomedical Sciences, Institute for Research in Biomedicine,Università della Svizzera italiana (USI), ²Graduate School of Cellular and Molecular Sciences, Faculty of Medicine,University of Bern, ³Institute of Computational Science,Università della Svizzera italiana (USI)

Capítulos

00:04Título
00:57Intranasal Influenza Infection and Neutrophil Isolation and Injection
03:21Surgical Preparation
05:04In Vivo Time Lapse Imaging
06:01Results: Representative Intravital 4D Imaging and Analyses
07:28Conclusion

Summary

Tadução automática

在本研究中, 我们提出了一个协议, 执行双光子活体成像和细胞相互作用分析的小鼠气管粘膜感染后流感病毒。这个协议将是相关的研究人员研究免疫细胞动力学在呼吸道感染。

Tags

Keywords: Two-photon Intravital Microscopy Immune Responses Airborne Pathogens Neutrophil Interactions Influenza Infection CD11c-YFP Mouse PR8 Inoculate Bone Marrow Percoll Gradient Neutrophil Isolation

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