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成年斑马鱼的口服插管: 一种评价生物活性化合物肠道吸收的模型

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds

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成年斑马鱼的口服插管: 一种评价生物活性化合物肠道吸收的模型

DOI:

10.3791/58366-v

•

04:33 min

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September 27, 2018

•

Jie Ji², Rosemary Thwaite², Nerea Roher²

¹Institute of Biotechnology and Biomedicine (IBB-UAB),Universitat Autònoma de Barcelona, ²Department of Cell Biology, Animal Physiology and Immunology,Universitat Autònoma de Barcelona

Capítulos

00:04Título
00:58Preparing the Equipment for Oral Intubation
01:22Zebrafish Anesthetization and Oral Intubation
02:40Zebrafish Intestine Dissection
03:22Results: Home-made Oral Intubation Device Successfully Delivers Fluorescent Nanoparticles IBs^TNFα with Low Average Percent Mortality
04:01Conclusion

Summary

Tadução automática

该协议描述插管成年斑马鱼与生物;然后对肠道进行解剖和制备, 用于细胞术、共焦显微镜和 qPCR。这种方法允许管理生物活性化合物, 以监测肠道摄取和局部免疫刺激诱发。对口腔预防手段的肠道动态检测有重要意义。

Tags

Oral Intubation Zebrafish Intestinal Uptake Bioactive Compounds Mucosa Vaccinology Nanoparticle Suspension Intestinal Dissection Anesthetizing Recovery Tank Euthanizing

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