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不同二硫 Connectivities µ-芋螺毒素 PIIIA 异构体的合成与结构测定
JoVE Journal
Química
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JoVE Journal Química
Synthesis and Structure Determination of µ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
DOI:

11:44 min

October 02, 2018

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Capítulos

  • 00:04Título
  • 00:49Solid-Phase Peptide Synthesis (SPPS), Peptide Cleavage from the Resin, and Purification of the Linear Precursor
  • 03:04Selective Formation of the Disulfide Bonds
  • 04:44Peptide Purification
  • 05:56Peptide Analytics
  • 08:04MS/MS Analysis of Disulfide Connectivity
  • 09:49Results: The Sequential Walk and the Resulting NMR Solution Structure of a Rigid and a Flexible -PIIIa Isomer
  • 10:30Conclusion

Summary

Tadução automática

富含半胱氨酸的肽折叠成不同的三维结构, 这取决于它们的二硫化物连通性。当缓冲氧化不导致所需的二硫化物连接时, 需要有针对性地合成单独的二硫化物异构体。该协议涉及 3-二硫键结合肽的选择性合成及其结构分析, 采用核磁共振和 ms/质谱研究。

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