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福尔马林固定癌组织标本免疫肿瘤学自动复合免疫荧光小组研究

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Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

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福尔马林固定癌组织标本免疫肿瘤学自动复合免疫荧光小组研究

DOI:

10.3791/58390-v

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10:49 min

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January 21, 2019

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Michael Surace, Karma DaCosta, Anna Huntley, Weiguang Zhao, Christopher Bagnall, Charles Brown, Chichung Wang, Kristin Roman, Jennifer Cann, Arthur Lewis, Keith Steele, Marlon Rebelatto, Edwin R. Parra, Clifford C. Hoyt, Jaime Rodriguez-Canales

¹Laboratory of Pathology,MedImmune, ²Laboratory of Pathology,MedImmune, ³Akoya Biosciences Inc., ⁴Department of Translational Molecular Pathology,University of Texas – MD Anderson Cancer Center

Capítulos

00:04Título
01:17Multiplex Immunofluorescence (mIF) Protocol Program Creation
03:09Reagent Preparation
05:19Multispectral Imaging
06:45Spectral Unmixing, Fluorescence Intensity, and Signal Attenuation
08:15Results: Representative mIF Imaging
09:59Conclusion

Summary

Tadução automática

对6个标记多重免疫荧光面板的详细协议进行了优化和执行, 使用自动着色器实现更一致的结果和更短的手术时间。这种方法可以直接由任何实验室用于免疫肿瘤学研究。

Tags

Multiplex Immunofluorescence Immuno-oncology Automated Staining Protocol Optimization Immune Cell Phenotyping Prognostic And Predictive Biomarkers Spectral Imaging Peroxidase Inhibition Primary And Secondary Antibodies Tyramide Signal Amplification Antigen Retrieval Drop Controls Isotype Controls

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