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Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models
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JoVE Journal Pesquisa do Câncer
Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models

Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models

DOI:
10.3791/58397-v

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08:43 min

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September 18, 2018

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, , ,
1Department of Urology,SUNY Upstate Medical University, 2Department of Biochemistry and Molecular Biology,SUNY Upstate Medical University, 3Laboratory of Comparative Pathology,Memorial Sloan-Kettering Cancer Center, 4Boulder BioPATH, Inc

Capítulos

  • 00:04TItle
  • 00:35Urogenital System (UGS) Dissection
  • 02:04Prostate Gross Anatomy and Individual Lobe Microdissection
  • 02:51Tissue Processing for 3D Culture
  • 04:41Cell Plating and Culture and Sphere Harvest
  • 06:38Results: Representative Mouse Prostate Lobe Analyses
  • 07:41Conclusion

Summary

Tadução automática

Tadução automática

Genetically engineered mice are useful models for investigating prostate cancer mechanisms. Here we present a protocol to identify and dissect prostate lobes from a mouse urogenital system, differentiate them based on histology, and isolate and culture the primary prostate cells in vitro as spheroids for downstream analyses.

Tags

Keywords: ProstateMouse ModelLobesSpheroid CultureIn Vitro AnalysisGenetically-engineeredProstate CancerUrogenital SystemFat RemovalSeminal VesiclesDorsal LobesLateral LobesVentral LobesAnterior LobesDMEM

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