Journal
/
Pesquisa do Câncer
/
基于荧光素酶的大抑癌 (拉图) 生物传感器监测河马信号通路活动
JoVE Journal
Pesquisa do Câncer
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Pesquisa do Câncer
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor
Please note that all translations are automatically generated. Click here for the English version.

基于荧光素酶的大抑癌 (拉图) 生物传感器监测河马信号通路活动

DOI:
10.3791/58416-v

•

07:16 min

•

September 13, 2018

•

, , , ,
1Department of Pathology and Molecular Medicine,Queen’s University

Capítulos

  • 00:04Título
  • 00:50Investigation of a Putative Regulator of Hippo Signaling Using the LATS-BS
  • 04:07A Screen to Identify Hippo Pathway Regulators Using LATS-BS
  • 05:50Results: LATS-BS Utilized to Measure Activation and Inhibition Effect of a Small Molecule on LATS Kinase Activity
  • 06:22Conclusion

Summary

Tadução automática

Tadução automática

在这里, 我们提出一个基于荧光素酶的生物传感器, 以量化的激酶活性的大抑癌 (拉图)-一个中心激酶在河马信号通路。该生物传感器在基础和平移研究中有不同的应用, 目的是在体外和体内对河马通路调节器进行调查。

Tags

Hippo Signaling PathwayLATS BiosensorLuciferaseBreast CancerLung CancerCellular Signaling NetworksIn VitroIn VivoTransfectionPassive Lysis BufferLuciferase AssayPositive ControlMSD

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58416?article_type=v

小鼠原位膀胱肿瘤模型和肿瘤检测系统
check_url/pt/58416?article_type=v

用医用金属丝捕获循环肿瘤细胞的肿瘤细胞: 基于免疫荧光和 DNA 的3D 方法
check_url/pt/58416?article_type=v

利用小鼠脑肿瘤球的原发染色质沉淀
check_url/pt/58416?article_type=v

腺病毒报告系统 TGF β/Smad3 信号通路的活体细胞成像
check_url/pt/58416?article_type=v

数字聚合酶链反应法测定零星家族性腺瘤性息肉病患者的基因变异
check_url/pt/58416?article_type=v

三维肿瘤球体的纵向形态和生理监测
check_url/pt/58416?article_type=v

利用血脑肿瘤屏障在盘中进行活体有效载荷传递预测
check_url/pt/58416?article_type=v

患者生物流体中肿瘤相关循环DNA的检测与监测
check_url/pt/58416?article_type=v

使用一套实用可行的方法,描述英国皇家空军家族激酶与疾病相关的突变体
check_url/pt/58416?article_type=v

使用阵列库的中等通量筛选和基于双 Luciferase 的分录报告器识别转录因子稳压器

Read Article