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Høj overførselshastighed måling af Dictyostelium discoideum Macropinocytosis af Flow flowcytometri
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Biologia
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JoVE Journal Biologia
High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

Høj overførselshastighed måling af Dictyostelium discoideum Macropinocytosis af Flow flowcytometri

DOI:
10.3791/58434-v

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06:47 min

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September 10, 2018

•

,
1MRC-Laboratory of Molecular Biology, Cambridge, UK

Capítulos

  • 00:04Título
  • 00:31Measuring Fluid Uptake
  • 02:51Performing Fluid Uptake Time-courses
  • 03:57Dose Response Curves
  • 05:09Results: Measurements of Dictyostelium discoideum Macropinocytosis
  • 06:25Conclusion

Summary

Tadução automática

Tadução automática

Macropinocytosis, store uspecifikke væske optagelse, er vigtige i mange områder af klinisk biologi herunder immunologi, infektion, kræft og neurodegenerative sygdomme. Her, er eksisterende teknikker blevet tilpasset til at give høj overførselshastighed, encellede opløsning måling af macropinocytosis i macropinocytosis model organisme Dictyostelium discoideum benytter flow flowcytometri.

Tags

Keywords: Dictyostelium DiscoideumMacropinocytosisFlow CytometryHigh-throughput MeasurementCell CultureTRITC-dextranCell WashingCell CountingTime CourseSodium Azide

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