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Immunization of Adult Zebrafish for the Preclinical Screening of DNA-based Vaccines
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Capítulos
- 00:04Título
- 00:35Pulling the Microinjection Needles
- 01:10Filling the Micropipette Needles
- 01:49Setting the Microinjector and Electroporator for Immunization
- 02:40Injection of the DNA Vaccine and Electroporation and Visualization of Antigen Expression
- 04:04Results: Danio rerio Injected with DNA-based Vaccine
- 05:04Conclusion
Here we describe a protocol for the immunization of the adult zebrafish (Danio rerio) with a DNA-based vaccine and demonstrate the validation of a successful vaccination event. This method is suitable for the preclinical screening of vaccine candidates in various infection models.
