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1Lab of Animal Models and Functional Genomics (LAMFG), The Key Laboratory of Animal Vaccine & Protein Engineering, College of Veterinary Medicine,Hunan Agricultural University (HUNAU), 2Department of Pathology,Georgetown University Medical School, 3College of Food Science and Technology,Hunan Agricultural University (HUNAU), 4Lab of Molecular Cardiology (LMC),National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH), 5Transgenic Core,National Heart, Lung, and Blood Institute (NHLBI)/National Institutes of Health (NIH)
Capítulos
- 00:04Título
- 01:02Southern Blotting Screening
- 04:40Results: Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells
- 06:07Conclusion
Här presenterar vi ett detaljerat protokoll för att identifiera homolog rekombination händelser som inträffat i mus embryonala stamceller använder södra blotting eller PCR. Denna metod exemplifieras av generationen av nonmuscle myosin II genetisk utbyte musmodeller med traditionella embryonala stamceller-baserade homolog rekombination-medierad inriktningsteknik.
