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Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy
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Bioquímica
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JoVE Journal Bioquímica
Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy

Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy

DOI:
10.3791/58475-v

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06:27 min

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November 30, 2018

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, , , , ,
1Patricia and John Rosenwald Laboratory of Neurobiology and Genetics,Rockefeller University, 2Electron Microscopy Resource Center,Rockefeller University

Capítulos

  • 00:04Título
  • 01:00Clot Turbidity Assay and Scanning Electron Microscopy
  • 04:11Results: Representative In Vitro Fibrin Clot Formation Analyses
  • 05:54Conclusion

Summary

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Presented here are two methods that can be used individually or in combination to analyze the effect of beta-amyloid on fibrin clot structure. Included is a protocol for creating an in vitro fibrin clot, followed by clot turbidity and scanning electron microscopy methods.

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Keywords: β-AmyloidFibrin ClotScanning Electron MicroscopyTurbidity AssayFibrinogenThrombinClot StructureProtein InteractionsInhibitors

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