/
/
Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer’s Patches
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:04Título
- 00:40Peyer’s Patch (PP) Harvest and Single Cell Suspension Preparation
- 04:08Surface Marker and Intracellular Protein Staining
- 06:23Results: Representative Isolated PP Lymphocyte Population Analyses
- 08:04Conclusion
In this study, we present a novel and effective protocol for the isolation of lymphocytes from Peyer's Patches (PPs), which can be subsequently used for in vivo and in vitro functional assays as well as flow cytometric studies of follicular T helper and germinal center B cells.
