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En Syngeneic mus B-celle lymfom modell for pre-klinisk evaluering av CD19 bil T-celler
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
A Syngeneic Mouse B-Cell Lymphoma Model for Pre-Clinical Evaluation of CD19 CAR T Cells
DOI:

12:16 min

October 16, 2018

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Capítulos

  • 00:04Título
  • 00:55Days 1 & 2: Preparation and Transfection of Plat-E Retroviral Packaging Cell Line
  • 02:11Day 3: Preparation of Virus-Containing Supernatant for Transduction and Isolation and In Vitro Activation of Mouse Splenic T Cells
  • 04:27Day 4: Transduction of Mouse T Cells
  • 06:43Day 5: Round 2 of Transduction
  • 08:33In Vitro Validation of CAR T Cell Activity
  • 09:53Results: Pre-Clinical Evaluation of CD19 CAR T Cells
  • 11:32Conclusion

Summary

Tadução automática

Her presenterer vi en protokoll for produksjon og pre-klinisk testing av murine CD19 bil T celler ved retroviral signaltransduksjon og utnyttelse som en behandling mot etablerte syngeneic A20 B-celle lymfom i BALB/c mus med eller uten lymphodepleting pre condition.

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