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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
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Bioquímica
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JoVE Journal Bioquímica
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

DOI:
10.3791/58542-v

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09:12 min

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January 18, 2019

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, , , , , , , , ,
1Department of Nephrology, Medical Faculty,Heinrich-Heine-University, 2Institute of Cellular and Integrative Physiology,University Medical Center Hamburg-Eppendorf

Capítulos

  • 00:04Título
  • 00:47Surgical Procedure
  • 03:00In Vivo Biotinylation and Glomeruli Isolation
  • 05:29Glomeruli Washing and Quantification
  • 06:25Results: Representative In Vivo Labeling of Glomerular Cell Surface Proteins
  • 08:31Conclusion

Summary

Tadução automática

Tadução automática

Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.

Tags

Keywords: Glomeruli IsolationIn Vivo LabelingGlomerular Cell Surface ProteinsProteinuric Kidney DiseaseCell Surface Protein TraffickingOrgan PerfusionKidney IsolationAortic PerfusionRenal VeinPBS Perfusion

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