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Visualisering og sporing endogene mRNAs i live Drosophila melanogaster egg Chambers
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Biologia
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JoVE Journal Biologia
Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

Visualisering og sporing endogene mRNAs i live Drosophila melanogaster egg Chambers

DOI:
10.3791/58545-v

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07:39 min

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June 04, 2019

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, , ,
1Biological Sciences Department Hunter College,City University of New York, 2Program in Molecular, Cellular, and Developmental Biology,Graduate Center, City University of New York

Capítulos

  • 00:04Título
  • 00:51Molecular Beacon (MB) Nurse Cell Microinjection
  • 02:38Tracking and Colocalization Analyses and Video File Preparation
  • 05:58Results: Representative MB Visualization and Tracking
  • 07:15Conclusion

Summary

Tadução automática

Tadução automática

Her presenterer vi en protokoll for visualisering, deteksjon, analyse og sporing av endogene mRNA smugling i live Drosophila melanogaster egg kammer ved hjelp av molekylære beacons, spinning plate konfokalmikroskopi mikroskopi, og åpen kildekodeanalyse Programvare.

Tags

MRNADrosophila MelanogasterEgg ChambersRNA LocalizationLive ImagingMolecular BeaconsMicroinjectionRNA BiologyReal-time VisualizationRNA TraffickingTherapeutic TargetsZebrafishCell CultureMicroscopyImage Analysis

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