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Et fluorescens udsving spektroskopi Assay af Protein-Protein interaktioner på celle-celle kontakter
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Capítulos
- 00:04Título
- 00:49Sample Preparation: Cell-Cell Mixing Assay
- 02:37Scanning Fluorescence Cross-Correlation Spectroscopy (sFCCS): Acquisition
- 04:07Scanning Fluorescence Cross-Correlation Spectroscopy (sFCCS): Data Analysis
- 06:03Results: Investigation of Protein-Protein Interactions at Cell-Cell Contacts by sFCCS
- 08:09Conclusion
Denne protokol beskriver et fluorescens udsving spektroskopi-baseret tilgang til at undersøge interaktioner mellem protein mægle celle-celle interaktioner, dvs protein lokaliseret i celle vejkryds, direkte i levende celler. Vi leverer detaljerede retningslinjer på instrument kalibrering, dataopsamling og analyse, herunder rettelser til mulige artefakt kilder.
Tags
Fluorescence Fluctuation SpectroscopyProtein-protein InteractionsCell-cell ContactsCell AdhesionCell RecognitionLiving CellsMono-membrane VesiclesModel OrganismsCell TransfectionGreen Fluorescent ProteinYellow Fluorescent ProteinRed Fluorescent ProteinEDTACell DetachmentCell ResuspensionLaser Scanning Confocal MicroscopeSpectral CrosstalkMEGFPMEYFPMCherryMCardinal
