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A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains
JoVE Journal
Neurociência
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JoVE Journal Neurociência
A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains

A Micro-CT-based Method for Characterizing Lesions and Locating Electrodes in Small Animal Brains

DOI:
10.3791/58585-v

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05:12 min

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November 08, 2018

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, , , , ,
1Department of Molecular and Cellular Biology,Harvard University, 2Center for Brain Science,Harvard University, 3Department of Organismic and Evolutionary Biology,Harvard University, 4Institute of Science and Technology Austria

Capítulos

  • 00:04Título
  • 00:57Post-fixation and Staining
  • 02:02Embedding and micro-CT
  • 03:18Results: Whole Brain micro-CT Imaging Used to Quantify Lesions and Electrodes in Small Animal Brains
  • 04:21Conclusion

Summary

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This article describes a straightforward method to prepare small animal brains for micro-CT imaging, in which lesions can be quantified and electrodes located with high precision in the context of the whole brain.

Tags

Micro-CTLesionsElectrodesSmall Animal BrainsNeurociência3D VolumeBrainProfusion SolutionOsmium TetroxideDehydrationResin InfiltrationMicro-CT Imaging

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