Journal
/
/
En rotte Methyl-Seq Platform til at identificere epigenetiske ændringer forbundet med Stress eksponering
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
DOI:

09:06 min

October 24, 2018

, , , , , , ,

Capítulos

  • 00:04Título
  • 01:04Sample Preparation
  • 01:54Hybridization
  • 04:16Bisulfite Conversion
  • 05:29Indexing
  • 06:06Validation by Bisulfite Pyrosequencing
  • 07:18Results: Stress-associated DNA Methylation Changes are verified by Bisulfite Pyrosequencing
  • 08:03Conclusion

Summary

Tadução automática

Vi beskriver her, protokol og gennemførelsen af Methyl-FF., en epigenomiske platform, ved hjælp af en rotte model til at identificere epigenetiske ændringer forbundet med kronisk stress eksponering. Resultaterne viser, at rotte Methyl-Seq platform er i stand til at opdage methylering forskelle, der opstår fra stress eksponering i rotter.

Vídeos Relacionados

Read Article