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h3 和 h4 组蛋白的纯化及细胞和脑组织中乙酰化组蛋白标记的定量
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
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h3 和 h4 组蛋白的纯化及细胞和脑组织中乙酰化组蛋白标记的定量

DOI:
10.3791/58648-v

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09:43 min

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November 30, 2018

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, ,
1Department of Psychiatry & Behavioral Sciences,University of Miami Miller School of Medicine, 2Center for Therapeutic Innovation,University of Miami Miller School of Medicine

Capítulos

  • 00:04Título
  • 01:06Preparation of the Sample Extract
  • 02:38Preparation of the Crude Histone Extract
  • 03:33Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract
  • 04:14Purification of Core Histones
  • 05:13Precipitation of Core Histones
  • 07:02Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain
  • 09:02Conclusion

Summary

Tadução automática

Tadução automática

本文的目的是为组蛋白 h3 和 h4 的有效纯化以及乙酰化组蛋白残留量的定量提供全面、系统的指导。

Tags

Histone ProteinsH3H4AcetylationPost-translational ModificationsPurificationQuantificationCell ExtractsAlzheimer’s DiseaseEpigenetic RegulationCell CultureCentrifugationBuffer ExtractionPH Neutralization

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