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Purification de H3 et H4 histones et la Quantification des histones acétylées marques dans les cellules et les tissus cérébraux
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Purification of H3 and H4 Histone Proteins and the Quantification of Acetylated Histone Marks in Cells and Brain Tissue
DOI:

09:43 min

November 30, 2018

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Capítulos

  • 00:04Título
  • 01:06Preparation of the Sample Extract
  • 02:38Preparation of the Crude Histone Extract
  • 03:33Evaluation of the Presence of Histone and/or Non-histone Protein in Histone Extract
  • 04:14Purification of Core Histones
  • 05:13Precipitation of Core Histones
  • 07:02Results: Human Microglial BV2 Histone Purified and Quantification in Wild Type and Transgenic Mouse Brain
  • 09:02Conclusion

Summary

Tadução automática

Le but de cet article est de fournir un guide complet et systématique à l’épuration efficace des histones H3 et H4 et la quantification des résidus d’histones acétylées.

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