Journal
/
Genética
/
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

DOI:
10.3791/58655-v

•

10:55 min

•

March 08, 2019

•

, , , , ,
1The Interdisciplinary Nanoscience Centre,Aarhus University, 2Department of Molecular Biology and Genetics,Aarhus University

Capítulos

  • 00:04Título
  • 00:33Cell Culture, and the Collection of EVs and RNA-associated Biomolecules
  • 02:54RNA Extraction and Quality Control
  • 04:20Library Construction and Quality Control
  • 09:04Results: Collecting exRNA from hMSC for Next Generation Sequencing
  • 10:31Conclusion

Summary

Tadução automática

Tadução automática

This protocol demonstrates how to purify extracellular microRNAs from cell culture media for small RNA library construction and next generation sequencing. Various quality control checkpoints are described to allow readers to understand what to expect when working with low input samples like exRNAs.

Tags

Extracellular MicroRNAsMesenchymal Stem CellsNext-generation SequencingExtracellular VesiclesRNA IsolationNanoparticle Tracking AnalysisUltracentrifugationQuality Control

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Read Article