Journal
/
Genética
/
人骨髓间充质干细胞细胞外微 rna 的分离、测序和分析
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
Please note that all translations are automatically generated. Click here for the English version.

人骨髓间充质干细胞细胞外微 rna 的分离、测序和分析

DOI:
10.3791/58655-v

•

10:55 min

•

March 08, 2019

•

, , , , ,
1The Interdisciplinary Nanoscience Centre,Aarhus University, 2Department of Molecular Biology and Genetics,Aarhus University

Capítulos

  • 00:04Título
  • 00:33Cell Culture, and the Collection of EVs and RNA-associated Biomolecules
  • 02:54RNA Extraction and Quality Control
  • 04:20Library Construction and Quality Control
  • 09:04Results: Collecting exRNA from hMSC for Next Generation Sequencing
  • 10:31Conclusion

Summary

Tadução automática

Tadução automática

该协议演示了如何从细胞培养培养基中纯化细胞外微 rna, 用于小型 rna 文库构建和下一代测序。描述了各种质量控制检查点, 以使读者了解在处理低输入样本 (如 exrna) 时的预期结果。

Tags

Extracellular MicroRNAsMesenchymal Stem CellsNext-generation SequencingExtracellular VesiclesRNA IsolationNanoparticle Tracking AnalysisUltracentrifugationQuality Control

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

工程人为因素来特异性地操纵人细胞中的选择性剪接

CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏

单缓步动物标本的超低输入基因组测序库制备

一种组合单细胞法表征人茎和祖种群的分子和免疫表型异质性

人牙小泡干细胞的分离、鉴定及微 rna 遗传修饰

CRISPR/Cas9 诱导人多潜能干细胞内源蛋白标记的应用

纳米测序全血 mRNA 测序

人类胰腺胰岛的单细胞RNA测序与分析

用于隔离和测序微RNA并使用开源工具分析微RNA的完整管道

在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰

Read Article