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Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo
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JoVE Journal Medicina
Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

DOI:
10.3791/58660-v

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09:36 min

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February 05, 2019

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, , , , , ,
1Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences and School of Medicine,Southeast University, 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering,Southeast University

Capítulos

  • 00:04Título
  • 01:00Polyethyleneimine-Coated Superparamagnetic Iron Oxide Nanoparticle (PEI-SPION) Preparation
  • 03:52PEI-SPION/small interfering (si) RNA Nanoparticle (NP) Agarose Gel Electrophoresis
  • 05:00In Vitro RAW264.7 Macrophages Transfection
  • 06:55Results: Representative Biological Characterization of PEI-SPION and PE-SPION/siRNA NPs
  • 08:51Conclusion

Summary

Tadução automática

Tadução automática

We describe a method of using polyethyleneimine (PEI)-coated superparamagnetic iron oxide nanoparticles for transfecting macrophages with siRNA. These nanoparticles can efficiently deliver siRNA to macrophages in vitro and in vivo and silence target gene expression.

Tags

Keywords: PolyethyleneimineIron Oxide NanoparticlesSPIONMacrophageDeliverySiRNAIn VitroIn VivoAllylic AcidDimercapto Acrylic AcidTriethylaminePHNanoparticle SynthesisCoatingInflammationCancer

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