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Capítulos
- 00:04Título
- 00:48Preparation of Bacterial Strains of Mutant Libraries
- 01:40Preparation of Gradient Dilutions and Mini-gradient Test
- 02:39Preparation of Discontinuous Density Gradients
- 03:39Adding Prepared Cells to Gradients and Separation by Centrifuge
- 04:30Results: Density Gradient Centrifugation Used to Separate Bacterial Strains Based on Capsule Production
- 05:10Conclusion
Vi demonstrere brugen af ikke-vedvarende tæthed gradienter at adskille bakteriel befolkning baseret på kapsel produktion. Denne metode bruges til at sammenligne kapsel beløb mellem kulturer, isolere mutanter med en specifik kapsel fænotype eller identificere kapsel regulatorer. Beskrevet her er optimering og drift af analysen.
