Journal
/
Genética
/
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

DOI:
10.3791/58682-v

•

07:50 min

•

November 21, 2018

•

, , ,
1Biochemistry Department,Boston University School of Medicine, 2Bioinformatic Hub,Boston University

Capítulos

  • 00:04Título
  • 00:34Dissection and Preparation of BAT for Chromatin Immunoprecipitation
  • 03:29Immune Complexes Collection and DNA Recovery
  • 05:51Results: ChIP Validation by qPCR
  • 07:17Conclusion

Summary

Tadução automática

Tadução automática

Here we describe a protocol for efficient chromatin immunoprecipitation (ChIP) followed by high-throughput DNA sequencing (ChIP-seq) of brown adipose tissue (BAT) isolated from a mouse. This protocol is suitable for both mapping histone modifications and investigating genome-wide localization of non-histone proteins of interest in vivo.

Tags

Chromatin ImmunoprecipitationChIPMouseFormaldehyde Cross-linkingCell LysisSonicationImmunoprecipitationAntibodyProtein A Agarose

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58682?article_type=v

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
check_url/pt/58682?article_type=v

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
check_url/pt/58682?article_type=v

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
check_url/pt/58682?article_type=v

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis
check_url/pt/58682?article_type=v

Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
check_url/pt/58682?article_type=v

Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
check_url/pt/58682?article_type=v

Chromatin Immunoprecipitation Assay Using Micrococcal Nucleases in Mammalian Cells
check_url/pt/58682?article_type=v

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)
check_url/pt/58682?article_type=v

High-Resolution Mapping of Protein-DNA Interactions in Mouse Stem Cell-Derived Neurons using Chromatin Immunoprecipitation-Exonuclease (ChIP-Exo)
check_url/pt/58682?article_type=v

Isolation of Adipose Tissue Nuclei for Single-Cell Genomic Applications

Read Article