Journal
/
Imunologia e Infecção
/
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

DOI:
10.3791/58689-v

•

09:40 min

•

November 28, 2018

•

, ,
1Virus and Prion Research Unit, National Animal Disease Center,USDA, Agricultural Research Service, 2ORAU/ORISE

Capítulos

  • 00:04Título
  • 00:48Processing of Swine Blood Samples and Organic Extraction for Total RNA and Small RNA
  • 02:05Total RNA Isolation Procedure
  • 03:33Globin Reduction
  • 07:24Results: Expression Libraries of the Globin-depleted and Ribo-depleted Whole Blood Samples
  • 08:50Conclusion

Summary

Tadução automática

Tadução automática

Here, we present a protocol optimized for the processing of coding (mRNA) and non-coding (ncRNA) globin reduced RNA-seq libraries from a single whole blood sample.

Tags

RNA ClassesCoding RNANon-coding RNAWhole BloodHost-pathogen InteractionsRNAseqGlobin-reducedMRNAMiRNAIsolationCentrifugationLysisPhenol-chloroformEthanolFilter CartridgeWash Solution

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Depletion of Specific Cell Populations by Complement Depletion

A 1.5 Hour Procedure for Identification of Enterococcus Species Directly from Blood Cultures

Introducing Shear Stress in the Study of Bacterial Adhesion

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

Nucleocapsid Annealing-Mediated Electrophoresis (NAME) Assay Allows the Rapid Identification of HIV-1 Nucleocapsid Inhibitors

Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood

Generation of Human Monocyte-derived Dendritic Cells from Whole Blood

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Read Article