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Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
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1Department of Laboratory Medicine,AnSteel Group General Hospital, 2Cancer Science Institute of Singapore,National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine,National University of Singapore, 4Department of Haematology-Oncology,National University Health System
Capítulos
- 00:04Título
- 00:57Monoplex Tyramide-Based Immunofluorescence
- 03:22Monoplex Slide and Tissue Microarray (TMA) Scanning
- 04:36Data Analysis
- 06:28Results: Representative Mutiplexed Fluorescent Immunohistochemical (mf-IHC) Staining, Imaging, and Analysis
- 07:10Conclusion
Here we describe a protocol for multiplex fluorescent immunohistochemical staining and imaging for the simultaneous localization of multiple cancer-associated antigens in lymphoma. This protocol can be extended to the colocalization analysis of biomarkers within all tissue sections.
