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Capítulos
- 00:04Título
- 00:40L. monocytogenes-infected Mouse Dissection and Cardiac Perfusion
- 01:51Organ Harvest and Bacterial Burden Determination
- 04:00Results: Representative Bacterial Burdens in Infected Mouse Organs
- 04:22Conclusion
During infection, Listeria monocytogenes is capable of crossing the blood-brain barrier to colonize the brain. In this protocol, we demonstrate how to assess bacterial colonization of organs following infection of mice. A procedure to perform whole organ perfusion for specific determination of bacterial numbers in the brain parenchyma is provided.
