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细胞系定量免疫印迹作为常规组织样本中生物标志物蛋白定量免疫荧光定量的标准
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Biologia
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JoVE Journal Biologia
Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples
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细胞系定量免疫印迹作为常规组织样本中生物标志物蛋白定量免疫荧光定量的标准

DOI:
10.3791/58735-v

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09:58 min

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January 07, 2019

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, , ,
1Department of Pathology and Molecular Medicine,Queen’s University, 2Division of Cancer Biology and Genetics,Queen’s Cancer Research Institute, 3Queen’s Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine,Queen’s University

Capítulos

  • 00:04Título
  • 01:29Building a Cell-Line Tissue Microarray (TMA)
  • 03:37Sample Staining by Immunofluorescence
  • 04:27Quantitative Immunoblotting of Cell Lines
  • 07:48Results: Immunoblotting Ratios Strongly and Positively Correlated with Intensity Readings from Quantitative IF
  • 09:22Conclusion

Summary

Tadução automática

Tadução automática

我们描述了使用定量免疫印迹来验证免疫荧光组织学结合图像分析作为一种手段, 定量的蛋白质感兴趣的福尔马林固定, 石蜡嵌入 (ffpe) 组织样本。我们的研究结果证明了免疫荧光组织学在确定常规活检样本中生物标志物蛋白的相对数量方面的作用。

Tags

Keyword Extraction: Quantitative ImmunoblottingCell LinesImmunofluorescenceBiomarker ProteinsCancer PathologyImmunohistochemistryProtein QuantificationImmortalized Cell LinesMultiplexed ApproachPrimary Biopsy SamplesCentrifugationCell PelletPBSNBFAgarose Solution

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