Journal
/
Biologia
/
Effekten af fluorescerende proteiner på Fusion partnere ved hjælp af polyglutamin toksicitet Assays i gær
JoVE Journal
Biologia
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Biologia
Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Effekten af fluorescerende proteiner på Fusion partnere ved hjælp af polyglutamin toksicitet Assays i gær

DOI:
10.3791/58748-v

•

09:23 min

•

November 28, 2018

•

, , , ,
1Department of Anatomy and Cell Biology,University of Western Ontario, 2Department of Pathology and Laboratory Medicine,University of Western Ontario

Capítulos

  • 00:04Título
  • 00:40Preparing Cell Cultures
  • 02:25Spotting Assay
  • 03:36Quantification of Cell Growth in Liquid Culture
  • 04:49Fluorescent Microscopy
  • 05:32Dot Blot
  • 07:26Results: Effects of Fluorescent Proteins on PolyQ Toxicity and Aggregation in Yeast
  • 08:50Conclusion

Summary

Tadução automática

Tadução automática

Denne artikel beskriver protokoller for at vurdere effekten af fluorescerende proteiner på en sammenlægning og toksicitet af fejlfoldede polyglutamin udvidelse til hurtig evaluering af en nyligt uncharacterized fluorescerende proteiner inden for rammerne af fluorescerende journalister.

Tags

Keywords: Fluorescent ProteinsFusion PartnersPolyglutamine ToxicityYeastHTT Exon One25Q72QMonomeric Superfolder GFPGAL1 PromoterPolyQ FusionsOptical Density

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58748?article_type=v

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
check_url/pt/58748?article_type=v

Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System

check_url/pt/58748?article_type=v

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
check_url/pt/58748?article_type=v

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging
check_url/pt/58748?article_type=v

Vækstassays til Vurdere polyglutamine Toksicitet i gær
check_url/pt/58748?article_type=v

Identifikation af Protein Interagerende Partners Brug Tandem Affinitetsrensning
check_url/pt/58748?article_type=v

Analyse af SNARE-medieret membranfusion anvendelse af en enzymatisk Cellefusion Assay
check_url/pt/58748?article_type=v

Identifikation af Protein Interaction Partnere i pattedvrceller Brug SILAC-immunpræcipitations Kvantitative Proteomics
check_url/pt/58748?article_type=v

Undersøgelse Spredningen og toksicitet af Prion-lignende proteiner Brug af metazoan modelorganismen<em> C. elegans</em
check_url/pt/58748?article_type=v

Sammenligning affiniteten af ​​GTPase-bindende proteiner under anvendelse af kompetitive assays

Read Article