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In Vitro 逆に HIV 転写、スプライシングのエージェントの待機時間の高スループット
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
DOI:

07:18 min

January 22, 2019

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Capítulos

  • 00:04Título
  • 00:32Transfection of HEK293T Cells with Dual Color Reporter Construct
  • 02:01Treatment of Transfected HEK293T Cells with Latency Reversing Agents
  • 02:33Staining of Transfected Cells with Fixable Viability Dye for Flow Cytometry Analysis
  • 04:40EGFP and DsRed Measurements by Flow Cytometry and Data Analysis
  • 05:49Results: Assessment of Latency Reversing Agents on HIV Transcription and Splicing
  • 06:43Conclusion

Summary

Tadução automática

HIV の効率的な再活性化の機能評価および潜在的な proviruses のクリアランスのための高いスループット プロトコル説明および HIV 転写の介入の影響をテストおよび選択的スプライシングによって適用しました。LTR 駆動の転写に対するエージェントの逆転と融着接続の遅延の効果の代表的な結果を提供しています。

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