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Imunologia e Infecção
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Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

DOI:
10.3791/58768-v

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06:15 min

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December 20, 2018

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, , ,
1Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine,National Cheng Kung University, 3Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine,National Cheng Kung University

Capítulos

  • 00:04Título
  • 00:37Synchronization of C. elegans
  • 02:24C. elegans Survival Assay with Aeromonas
  • 03:22C. elegans Liquid Toxicity Assay with Aeromonas
  • 04:14C. elegans Muscle Necrosis Assay with Aeromonas
  • 04:57Results: Diversity in Survival Rates of C. elegans Infected with Aeromonas spp. Consistent with Clinical Observations
  • 05:45Conclusion

Summary

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Here, we introduce three different experiments to study Aeromonas infection in C. elegans. Using these convenient methods, it is easy to evaluate the toxicity among and within Aeromonas species.

Tags

AeromonasCaenorhabditis ElegansVirulencePathogenesisHost-pathogen InteractionToxicityPathogenicityL1 WormsL4 WormsNGM PlatesE. Coli OP50Bacterial BrothOD 600 Absorbance

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