Journal
/
Química
/
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
JoVE Journal
Química
This content is Free Access.
JoVE Journal Química
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

DOI:
10.3791/58771-v

•

08:30 min

•

January 19, 2019

•

,
1Molecular Diagnostics, Centre for Health and Bioresources,AIT Austrian Institute of Technology GmbH

Capítulos

  • 00:04Título
  • 01:12Polyplex Formation
  • 02:16Agarose Gel Electrophoresis
  • 03:12Stability of Polyplexes Towards DNase I
  • 04:36Testing the Addressability of Horseradish Peroxidase Enzyme (HRP)-functionalized DNA Origami (HRP-NR) Upon Polycation Coating
  • 05:44Results: Characterization of DNA Origami Polyplexes
  • 07:42Conclusion

Summary

Tadução automática

Tadução automática

Here, a protocol for the protection of DNA origami nanostructures in Mg-depleted and nuclease-rich media using natural cationic polysaccharide chitosan and synthetic linear polyethyleneimine (LPEI) coatings is presented.

Tags

Keywords: DNA OrigamiNanostructuresGene TherapyPolycation CoatingEnzymatic DegradationCellular UptakeGene DeliveryAgarose Gel Electrophoresis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/58771?article_type=v

Origami Inspired Self-assembly of Patterned and Reconfigurable Particles
check_url/pt/58771?article_type=v

Gyroid Nickel Nanostructures from Diblock Copolymer Supramolecules
check_url/pt/58771?article_type=v

Folding and Characterization of a Bio-responsive Robot from DNA Origami
check_url/pt/58771?article_type=v

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
check_url/pt/58771?article_type=v

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation
check_url/pt/58771?article_type=v

Bio-inspired Polydopamine Surface Modification of Nanodiamonds and Its Reduction of Silver Nanoparticles
check_url/pt/58771?article_type=v

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
check_url/pt/58771?article_type=v

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates
check_url/pt/58771?article_type=v

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
check_url/pt/58771?article_type=v

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas (Video) | JoVE

Read Article