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一种用汽车 t 细胞进行的切杀检测

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A Spheroid Killing Assay by CAR T Cells

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一种用汽车 t 细胞进行的切杀检测

DOI:

10.3791/58785-v

•

08:19 min

•

December 12, 2018

•

Pierre Dillard, Hakan Köksal, Else-Marit Inderberg, Sebastien Wälchli

¹Department of Cellular Therapy, Department of Oncology,Oslo University Hospital-Radiumhospitalet

Capítulos

00:04Título
00:31Colorectal Cancer Cell Line Spheroid Generation
03:113D Tumor Spheroid Killing Assay
05:00Automated Image Analysis
06:59Results: Representative Spheroid Killing Analysis
07:54Conclusion

Summary

Tadução automática

该方案旨在实时评估免疫治疗重定向 t 细胞 (car t 细胞) 对三维结构癌细胞 (球体) 的细胞毒性。

Tags

3D Tumor Cells CAR T Cells Cytotoxicity Assay Colorectal Cancer Spheroid Automated Imaging Time-lapse Microscopy Immunotherapy

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